TY  - JOUR
AU  - Martín, M.
AU  - Salazar, P.
AU  - Jiménez, C.
AU  - Lecuona, M.
AU  - Ramos, M.J.
AU  - Ode, J.
AU  - Alcoba, J.
AU  - Roche, R.
AU  - Villalonga, R.
AU  - Campuzano, S.
AU  - Pingarrón, J.M.
AU  - González-Mora, J.L.
T1  - Rapid Legionella pneumophila determination based on a disposable core-shell Fe3O4@poly(dopamine) magnetic nanoparticles immunoplatform
LA  - eng
PY  - 2015
SP  - 51
EP  - 58
T2  - Analytica Chimica Acta
SN  - 1873-4324
VL  - 887
PB  - Elsevier B.V.
AB  - A novel amperometric magnetoimmunoassay, based on the use of core-shell magnetic nanoparticles and screen-printed carbon electrodes, was developed for the selective determination of Legionella pneumophila SG1. A specific capture antibody (Ab) was linked to the poly(dopamine)-modified magnetic nanoparticles (MNPs@pDA-Ab) and incubated with bacteria. The captured bacteria were sandwiched using the antibody labeled with horseradish peroxidase (Ab-HRP), and the resulting MNPs@pDA-Ab-Legionella neumophila-Ab-HRP were captured by a magnetic field on the electrode surface. The amperometric response measured at -0.15 V vs. Ag pseudo-reference electrode of the SPCE after the addition of H2O2 in the presence of hydroquinone (HQ) was used as transduction signal. The achieved limit of detection, without pre-concentration or pre-enrichment steps, was 104 Colony Forming Units (CFUs) mL-1. The method showed a good selectivity and the MNPs@pDA-Ab exhibited a good stability during 30 days. The possibility of detecting L. pneumophila at 10 CFU mL-1 level in less than 3 h, after performing a membrane-based preconcentration step, was also demonstrated.
DO  - 10.1016/J.ACA.2015.05.048
UR  - https://portalciencia.ull.es/documentos/5d9dafb8299952484ef81de7
DP  - Dialnet - Portal de la Investigación
ER  -