TY - JOUR AU - Pérez-Francés, J.F. AU - Valdés, F. AU - Martín, R. T1 - Callus induction and culture from explants of Erysimum scoparium in a growth regulator-free medium LA - eng PY - 1995 SP - 223 EP - 228 T2 - Plant Cell, Tissue and Organ Culture SN - 0167-6857 VL - 43 IS - 3 PB - Kluwer Academic Publishers AB - Calli from cotyledon, hypocotyl and radicle explants of Erysimum scoparium (Brassicaceae) were induced and cultured using a MS medium without growth regulators. Calli obtained from cotyledon or hypocotyl explants showed a higher growth rate while radicle-derived calli exhibited poor growth. Cotyledon-derived calli were compact and green-colored while hypocotyl- and radicle-derived calli were friable and with creamy-green pigmentation. Histological analysis was carried out during culture and the development of abundant vascular elements and meristematic nodules were found. Subculture on a growth regulator-free medium during 10 months did not ecrease callus growth. © 1995 Kluwer Academic Publishers. DO - 10.1007/BF00039948 UR - https://portalciencia.ull.es/documentos/5e3adb32299952629a023841 DP - Dialnet - Portal de la Investigación ER -