TY  - JOUR
AU  - Cequier-Sánchez, E.
AU  - Rodríguez, C.
AU  - Ravelo, Á.G.
AU  - Zárate, R.
T1  - Dichloromethane as a solvent for lipid extraction and assessment of lipid classes and fatty acids from samples of different natures
LA  - eng
PY  - 2008
SP  - 4297
EP  - 4303
T2  - Journal of Agricultural and Food Chemistry
SN  - 0021-8561
VL  - 56
IS  - 12
AB  - The usefulness of the solvent mixture dichloromethane/methanol for lipid extraction and the determination of lipid classes and fatty acids in samples of different natures was conducted. Two different extraction methods were compared, one containing chloroform/methanol, another containing dichloromethane/ methanol. Total lipid extraction showed some minor differences but no variation in the lipid classes. Regarding the fatty acid profile, in Echium virescens seeds, 17 major fatty acids could be identified and quantified, and all were equally extracted when either solvent system was employed. In Echium acanthocarpum hairy roots, 17 major fatty acids were quantified, showing some statistical differences for one cell line in favor of chloroform. The data obtained from the liquid nutrient medium were also comparable. The cod roe sample showed 31 major fatty acids, showing no statistical differences between the two solvent systems. Contrarily, the CH2Cl2 method was able to extract 31 main fatty acids found in European seabass dorsal muscle more efficiently than the CHCl3 method. The results indicate that, for lipid extraction and fatty acid assessment, dichloromethane/methanol can readily replace the commonly employed chloroform/methanol, thus avoiding the major health, security, and regulatory problems associated with the use of chloroform. © 2008 American Chemical Society.
DO  - 10.1021/jf073471e
UR  - https://portalciencia.ull.es/documentos/5e3c34eb29995246bbf5d051
DP  - Dialnet - Portal de la Investigación
ER  -