TY  - JOUR
AU  - D'Orazio, G.
AU  - Hernández-Borges, J.
AU  - Asensio-Ramos, M.
AU  - Rodríguez-Delgado, M.A.
AU  - Fanali, S.
T1  - Capillary electrochromatography and nano-liquid chromatography coupled to nano-electrospray ionization interface for the separation and identification of estrogenic compounds
LA  - eng
PY  - 2016
SP  - 356
EP  - 362
T2  - Electrophoresis
SN  - 1522-2683
VL  - 37
IS  - 2
PB  - Wiley-VCH Verlag
AB  - Nano-LC and CEC were coupled to MS through a nanospray or a pressurized liquid-junction interface for the simultaneous separation and determination of 11 estrogenic compounds. Different stationary phases, that is, phenyl, C18, and C18 bidentate silica hydrate, were studied. For both techniques, the phenyl stationary phase was the best option, considering separation efficiency, selectivity, and resolution. Under the optimized conditions, the baseline separation of the target compounds (including estradiol and zearalanol epimers) was achieved in less than 20 min in nano-LC-MS and less than 13 min in CEC-MS. Molecular imprinted polymer SPE was used for extracting the target compounds from mineral water samples with the analysis of nano-LC-MS. The whole molecular imprinted polymer SPE nano-LC-MS method was validated through a recovery study at two levels of concentration. Sensitivity was improved by on-column focusing technique obtaining LODs in the range 1.4-55.4 ng/L.
DO  - 10.1002/ELPS.201500327
UR  - https://portalciencia.ull.es/documentos/5e3c3c5929995246bbf60710
DP  - Dialnet - Portal de la Investigación
ER  -