TY  - JOUR
AU  - Revenu, C.
AU  - Ubelmann, F.
AU  - Hurbain, I.
AU  - El-Marjou, F.
AU  - Dingli, F.
AU  - Loew, D.
AU  - Delacour, D.
AU  - Gilet, J.
AU  - Brot-Laroche, E.
AU  - Rivero, F.
AU  - Louvard, D.
AU  - Robine, S.
T1  - A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins
LA  - eng
PY  - 2012
SP  - 324
EP  - 336
T2  - Molecular Biology of the Cell
SN  - 1059-1524
VL  - 23
IS  - 2
AB  - Actin-bundling proteins are identified as key players in the morphogenesis of thin membrane protrusions. Until now, functional redundancy among the actin-bundling proteins villin, espin, and plastin-1 has prevented definitive conclusions regarding their role in intestinal microvilli. We report that triple knockout mice lacking these microvillar actin-bundling proteins suffer from growth delay but surprisingly still develop microvilli. However, the microvillar actin filaments are sparse and lack the characteristic organization of bundles. This correlates with a highly inefficient apical retention of enzymes and transporters that accumulate in subapical endocytic compartments. Myosin-1a, a motor involved in the anchorage of membrane proteins in microvilli, is also mislocalized. These findings illustrate, in vivo, a precise role for local actin filament architecture in the stabilization of apical cargoes into microvilli. Hence, the function of actin-bundling proteins is not to enable microvillar protrusion, as has been assumed, but to confer the appropriate actin organization for the apical retention of proteins essential for normal intestinal physiology. © 2012 Revenu et al.
DO  - 10.1091/mbc.E11-09-0765
UR  - https://portalciencia.ull.es/documentos/5ee0c11d2999527595f96228
DP  - Dialnet - Portal de la Investigación
ER  -