TY  - JOUR
AU  - Diosa, J.A.G.
AU  - Orive, A.G.
AU  - Grundmeier, G.
AU  - Amado, R.J.C.
AU  - Keller, A.
T1  - Morphological dynamics of leukemia cells on TiO2 nanoparticle coatings studied by AFM
LA  - eng
PY  - 2021
T2  - Applied Sciences (Switzerland)
SN  - 2076-3417
VL  - 11
IS  - 21
PB  - MDPI
AB  - Coatings of modified TiO2 nanoparticles (TiO2-m) have been shown to effectively and se-lectively trap non-adherent cancer cells, with an enormous potential for applications in photody-namic therapy (PDT). Leukemia cells have a remarkable affinity for TiO2-m coatings, adhering to the surface by membrane structures and exhibiting morphologic characteristics of amoeboid loco-motion. However, the details of the cell–substrate interaction induced by the TiO2-m coating remain elusive. With the aim to obtain a better understanding of this phenomenon, leukemia cell adhesion to such coatings was characterized by atomic force microscopy (AFM) for short contact times up to 60 min. The cell and membrane morphological parameters mean cell height, contact area, cell vol-ume, and membrane roughness were determined at different contact times. These results reveal cell expansion and contraction phases occurring during the initial stage of adhesion. Subsequently, the leukemic cells reach what appears to be a new resting state, characterized by pinning of the cell membrane by TiO2-m nanoparticle aggregates protruding from the coating surface.
DO  - 10.3390/APP11219898
UR  - https://portalciencia.ull.es/documentos/618608588a2f583ff09af3b1
DP  - Dialnet - Portal de la Investigación
ER  -