Desarrollo de una animación 3D sobre la microscopia de onda evanescente y su aplicación en virologíauna herramienta para el estudio y comprensión de los mecanismos de infección por microorganismos en célula viva

  1. Agustin Valenzuela Fernandez 1
  2. José David Machado Ponce 1
  3. David Reyes Zamudio 1
  1. 1 Universidad de La Laguna
    info

    Universidad de La Laguna

    San Cristobal de La Laguna, España

    ROR https://ror.org/01r9z8p25

Book:
De los procesos de cambio al cambio con sentido
  1. Ana Vega Navarro (coord.)

Publisher: Servicio de Publicaciones

ISBN: 978-84-16471-20-1

Year of publication: 2019

Pages: 223-234

Type: Book chapter

DOI: 10.25145/B.INNOVAULL.2019.016 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

Abstract

In the study of the mechanisms of invasion by pathogens (viruses, bacteria, parasites), it is important to address, at an advanced and molecular level, the processes that govern membrane fusion, as one of the key biological events in infection by microorganisms and in the context of Infectious Diseases. In this scenario, it is important to understand how the proteins of the pathogens that regulate the invasion of cells and tissues work, by controlling and promoting the process of membrane fusion between the pathogen and the target cell. In this matter, the use of evanescent wave microscopy (TIRFM) is key and very important. The scientific illustrations in this topic are usually fixed sheets or 2D-diagrams that illustrate the function of the proteins involved and the fusion of membranes between pathogen and target cell, with sequential chains of non-dynamic images, where it is difficult to illustrate how this last generation microscope works. In this chapter we briefly present our ULL teaching innovation project, whose objective has been the creation and generation, for the first time, of a 3D Animation material to illustrate in a pedagogical way, and with the greatest scientific rigor, the application of the evanescent wave fluorescence microscopy technology in the study of living cells and the mechanism of their infection by pathogens, applying it to the understanding of the membrane fusion process and, in particular, to the process of early infection by the HIV-1.