Multiplex PCR for simultaneous detection of enterococcal genes vanA and vanB and staphylococcal genes mecA, ileS-2 and femB

  1. Elena Ramos Trujillo 1
  2. Eduardo Pérez Roth 1
  3. Sebastián Méndez Álvarez 2
  4. Félix Claverie Martín 1
  1. 1 Hospital Universitario Nuestra Señora de Candelaria

    Hospital Universitario Nuestra Señora de Candelaria

    Santa Cruz de Tenerife, España

    GRID grid.411331.5

  2. 2 Universidad de La Laguna

    Universidad de La Laguna

    San Cristobal de La Laguna, España

    GRID grid.10041.34

International microbiology: official journal of the Spanish Society for Microbiology

ISSN: 1618-1905

Year of publication: 2003

Volume: 6

Issue: 2

Pages: 113-115

Type: Article

Export: RIS
DOI: 10.1007/s10123-003-0118-z DIALNET GOOGLE SCHOLAR lock_openOpen access editor


SCImago Journal Rank

  • Year 2003
  • SJR Journal Impact: 0.391
  • Best Quartile: Q2
  • Area: Microbiology (medical) Quartile: Q2 Rank in area: 42/99
  • Area: Microbiology Quartile: Q3 Rank in area: 70/108


The experimental transfer of the vanA gene cluster from Enterococcus faecalis to Staphylococcus aureus has raised fears about the occurrence of such genetic transfer in clinical isolates of methicillin-resistant staphylococci. Recently, infections by a S. aureus strain carrying the enterococcal vancomycin resistance vanA gene cluster were reported. The possible emergence and dissemination of these strains is a serious health threat and makes optimization of prevention strategies and fast detection methods absolutely necessary. In the present study, we developed a PCR protocol for simultaneous detection of enterococcal vanA and vanB genes, the staphylococcal methicillin and mupirocin resistance markers mecA and ileS-2, and identification of S. aureus. As no vancomycin-resistant S. aureus isolates were available for our study, we used mixtures of enterococcal and staphylococcal colonies that harbored the different resistance markers to show that these genes could be detected simultaneously. This protocol could be used to facilitate the detection and identification of predictable S. aureus or methicillin-resistant strains carrying vanA or vanB.