Bartonella species and haplotypes in rodents and their fleas in Lanzarote and El Hierro in the Canary Islands, Spain

  1. Martin‐Carrillo, Natalia 11
  2. Izquierdo‐Rodriguez, Elena 11
  3. Abreu‐Yanes, Estefanía 11
  4. Foronda, Pilar 11
  5. Abreu‐Acosta, Néstor
  1. 1 Universidad de La Laguna

    Universidad de La Laguna

    San Cristobal de La Laguna, España


Journal of Vector Ecology

ISSN: 1081-1710 1948-7134

Datum der Publikation: 2020

Ausgabe: 45

Nummer: 2

Seiten: 254-261

Art: Artikel

DOI: 10.1111/JVEC.12396 GOOGLE SCHOLAR lock_openOpen Access editor

Andere Publikationen in: Journal of Vector Ecology

Objetivos de desarrollo sostenible


Because isolated ecosystems contribute to species variability, especially oceanic island ecosystems, the present work focused on the study of the Bartonella species and haplotypes in Lanzarote and El Hierro, two Canary islands with evident bioclimatic dierences between them. A total of 123 rodents and 110 eas from two islands were screened for the presence of Bartonella by PCR analysis of the gltA and nuoG genes. e overall prevalence was 5.7% in rodents and 20.4% in eas. A total of seven gltA-haplotypes was found in both rodents and eas, belonging to the species Bartonella mastomydis and Bartonella tribocorum in Lanzarote, and to Bartonella rochalimae and Bartonella elizabethae in El Hierro, as well as recently described species Bartonella kosoyi in both islands. Besides, potential co-infections were detected based on the nuoG analysis. Further, Xenopsylla cheopis was the only ea species identied. Our study shows that isolated ecosystems such as the Canary Islands lead to the appearance of new Bartonella haplotypes along dierent biotopes, with diverse ea species involved in the spreading of the pathogen being of great relevance due to the zoonotic potential of the species found.

Bibliographische Referenzen

  • Abreu-Yanes, E., A. Martín-Alonso, N. Martín-Carrillo, K. García-Livia, A. Marrero-Gagliardi, B. Valladares, C. Feliú, and P. Foronda P. 2018. Bartonella in rodents and ectoparasites in the Canary Islands, Spain: new insights into host-vector-pathogen relationships. Microb. Ecol. 75: 264-291.
  • Altschul, S.F., W. Gish, W. Miller, E.W. Myers, and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
  • Bai, Y., A. Gilbert, K. Fox, L. Osikowicz, and M. Kosoy. 2016. Bartonella rochalimae and B. vinsonii subsp. Berkhoi in wild carnivores from Colorado, USA. J. Wildl. Dis. 52: 844-849.
  • Billeter, S.A., M.G. Levy, B.B. Chomel, and E.B. Breitschwerdt. 2008. Vector transmission of Bartonella species with emphasis on the potential for tick transmission. Med. Vet. Entomol. 22: 1-15.
  • Buffet, J.P., B. Pisanu, S. Brisse, S. Roussel, B. Félix, L. Halos, J.L. Chapuis, and M. Vayssier-Taussat. 2013. Deciphering Bartonella diversity, recombination, and host specicity in a rodent community. PloS One. 8: e68956.
  • Colborn, J.M., M.Y. Kosoy, V.L. Motin, M.V. Telepnev, G. Valbuena, K.S. Myint, Y. Fofanov, C. Putonti, C. Feng, and L. Peruski. 2010. Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG). J. Clin. Microbiol. 48: 4630-4633.
  • Dahmani, M., G. Diatta, N. Labas, A. Diop, H. Bassene, D. Raoult, L. Granjon, F. Fenollar, and O. Mediannikov. 2018. Non-contagious nished genome sequence and description of Bartonella mastomydis sp. nov. New Microbes New Infect. 25: 60-70.
  • Gundi, V., S. Billeter, M. Rood, and M. Kosoy. 2012. Bartonella spp. in rats and zoonoses, Los Ángeles, California, USA. Emerg. Infect. Dis. 18: 631-633.
  • Gutiérrez, R., T. Shalit, B. Markus, C. Yuan, Y. Nachum-Biala, D. Elad, and S. Harrus. 2020. Bartonella kosoyi sp. nov. and Bartonella krasnovii sp. nov., two novel species closely related to the zoonotic Bartonella elizabethae, isolated from black rats and wild desert rodent-flea. Int. J. Syst. Evol. Microbiol. 70: 1656-1665.
  • Halliday, J.E., D.L. Knobel, B. Agwanda, Y. Bai, R.F. Breiman, S. Cleaveland, K. Njenga, and M. Kosoy. 2015. Prevalence and diversity of small mammal-associated Bartonella species in rural and urban Kenya. PLoS Negl. Trop. Dis. 9: e0003608.
  • Hayman, D., K. McDonald, and M. Kosoy. 2013. Evolutionary history of rat-borne Bartonella: the importance of commensal rats in the dissemination of bacterial infections globally. Ecol. Evol. 3: 3195-3203.
  • Jiyipong, T., S. Jittapalapong, S. Morand, and J.M. Rolain. 2014. Bartonella species in small mammals and their potential vectors in Asia. Asian Pac. J. Trop. Biomed. 4: 757-767.
  • Kandelaki, G., L. Malania, Y. Bai, N. Chakvetadze, G. Katsitadze, P. Imnadze, C. Nelson, S. Harrus, and M. Kosoy. 2016. Human lymphadenopathy caused by ratborne Bartonella, Tbilisi, Georgia. Emerg. Infect. Dis. 22: 544-546.
  • Kosoy, M.Y., D.T. Hayman, and K.S. Chan. 2012. Bartonella bacteria in nature: where does population variability end and species start? Infec. Genet. Evol. 12: 894-904.
  • Kosoy, M., C. McKee, L. Albayrak, and Y. Fofanov. 2017. Genotyping of Bartonella bacteria and their animal hosts: current status and perspectives. Parasitology 145: 543-562
  • Kumar, S., G. Stecher, M. Li, C. Knyaz, and K. Tamura. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol. Biol. Evol. 35: 1547-1549.
  • Lin, J.W., Y.M. Hsu, B.B. Chomel, L.K. Lin, J.C. Pei, S.H. Wu, and C.C. Chang. 2012. Identication of novel Bartonella spp. in bats and evidence of Asian gray shrew as a new potential reservoir of Bartonella. Vet. Microbiol. 156: 119-126.
  • López, C., S. Clemente, C. Almeida, A. Brito, and M. Hernández. 2015. A genetic approach to the origin of Millepora sp. in the eastern Atlantic. Coral Reefs 34: 631-638.
  • Martín-Alonso, A., G. Houemenou, E. Abreu-Yanes, B. Valladares, C. Feliú, and P. Foronda. 2016. Bartonella spp. in small mammals, Benin. Vector Borne Zoonotic Dis. 16: 229-237.
  • Mediannikov, O., K. El-Karkouri, C. Robert, P.E. Fournier, and D. Raoult. 2013. Non-contagious nished genome sequence and description of Bartonella orenciae sp. nov. Stand. Genomic Sci. 9: 185-196.
  • Meddiannikov, O., M. Aubadie, H. Bassene, G. Diatta, L. Granjon, and F. Fenollar. 2014. Three new Bartonella species from rodents in Senegal. Int. J. Infect. Dis. 21: 335.
  • Miceli, N.G., F.A. Gavioli, L.R. Gonçalves, M.R. André, V.R. Sousa, K.C. Sousa, and R.Z. Machado. 2013. Molecular detection of feline arthropod-borne pathogens in cats in Cuiaba, state of Mato Grosso, central-western region of Brazil. Rev. Bras. Parasitol. Vet. 22: 385-390.
  • Morick, D., G. Baneth, B. Avidor, M.Y. Kosoy, K.Y. Mumcuoglu, D. Mintz, O. Eyal, R. Goethe, A. Mietze, N. Shpigel, and S. Harrus. 2009. Detection of Bartonella spp. in wild rodents in Israel using HRM real-time PCR. Vet. Microbiol. 139: 293-297.
  • Myers, N., R.A. Mittermeier, C.G. Mittermeier, G.A. da Fonseca, and J. Kent. 2000. Biodiversity hotspots for conservation priorities. Nature 403: 853-858.
  • Nei, M. and S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford University Press.
  • Norman, A.F., R. Regnery, P. Jameson, C. Greene, and D.C. Krause. 1995. Diferentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene. J. Clin. Microbiol. 33: 1797-1803.
  • Paziewska, A., E. Sinski, and P.D. Harris. 2012. Recombination, diversity and allele sharing of infectivity proteins between Bartonella species from rodents. Microb. Ecol. 64: 525-536.
  • Saitou, N. and M. Nei. 1987. e neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
  • Whiting, M.F. 2002. Mecoptera is paraphyletic: multiple genes and phylogeny of Mecoptera and Siphonaptera. Zool. Scr. 31: 93-104.
  • Zurita, A., S.C. Gutiérrez, and C. Cutillas. 2016. Infection rates of Wolbachia sp. and Bartonella sp. in dierent populations of eas. Curr. Microbiol. 73: 704-713