Medicinal plant Helychrisum stoechas and endophytic fungi: hints of ecology.
- Laia Guardia
- Samuel Rodríguez Sabina 1
- Andreea Cosoveanu
- Raimundo Manuel Cabrera Pérez 1
-
1
Universidad de La Laguna
info
ISSN: 2248-129X, 2248-129X
Ano de publicación: 2019
Volume: 12
Páxinas: 35-40
Tipo: Artigo
Outras publicacións en: Romanian Journal for Plant Protection
Resumo
The genus Helichrysumis a valuable source of essential oils with medicinal properties. Eighty endophytic fungi have been isolated from 8 Helichrysum stoechasplants belonging to two different biotypes of Catalonia. Based on their morphology, 10 different genera have been identified while otherhave been assigned to 21 OTUs. The colonization rate was similar in bothlocations (71.53% and 67.36%) and in the entire plants (60-75%).In plant organs values ranged between 50 and 91.67.The PDA wasthe nutrient medium which provided the highest number of different colonies considering allthree media used (PDA, MEA and LCA). The colonization frequency index showeda tendency of the genera and OTUs to specificity of a single organ (21 OTUs and genera were obtained from a single organ, 8 from two organs and only 1 from all the organs used), and nutrientmedium (18 OTUs and genera were isolated in a single nutrient medium, 9 in two nutrientmedia and only 3 in all media used). The most frequent genera / OTUs were Alternariaand OTU1.
Referencias bibliográficas
- COSOVEANU A., Da SILVA E., GIMENEZ MARINO C., NUNEZ TRUJILLO G., GONZALES COLOMA A., FRIAS VIERA I., CABRERA R. (2012). Artemisia thusculaCav.: Antibacterial, Antifungal Activity of the Plant Extracts and Associated Endophytes. The Journal of Horticultural Science and Biotechnology, 16, 87-90.
- COSOVEANU, A., NITA, C.E., IACOMI, B.M., SABINA, S.R., CABRERA, R. (2016b). Active fungal endophytes against phytopathogenic fungi-dwellers of Romanian and Canarian Artemisia spp. Scientific Papers. Series B, Horticulture, LX, 291-298.
- COSOVEANU, A., SABINA, S.R.,CABRERA, R. (2018). Fungi as endophytes in Artemisia thuscula: juxtaposed elements of diversity and phylogeny. Journal of Fungi, 4, 17.
- GIULIANI, C., LAZZARO, L., CALAMASSI, R., CALAMAI, L., ROMOLI, R., FICO, G., FOGGI, B.,LIPPI, M.M. (2016). A volatolomic approach for studying plant variability: the case of selected Helichrysum species (Asteraceae). Phytochemistry,130, 128-143.
- KHAN, R., SHAHZAD, S., IQBALCHOUDHARY, M., KHAN, S.A.,AHMAD, A. (2010). Communities of endophytic fungi in medicinal plant Withania somnifera. Pakistan Journal of Botany,42, 1281-1287.
- LEONARDI, M., AMBRYSZEWSKA, K.E., MELAI, B., FLAMINI, G., CIONI., P.L., PARRI, F. & PISTELLI, L. (2013). Essential-Oil Composition of Helichrysum italicum(Roth) G.Don ssp. italicum from Elba Island (Tuscany, Italy). Chemistry & Biodiversity, 10,343-355.
- MASALLES, R. M. (1988). Plantes superiors. In: FOLCH, R. (Ed.),Història Natural dels Països Catalans, vol. 6, Enciclopèdia Catalana S. A., Barcelona.
- OSONO, T., ISHII, Y., TAKEDA, H., SERAMETHAKUN, T., KHAMYONG, S., TO-ANUN, C., HIROSE, D., TOKUMASU, S.,KAKISHIMA, M. (2009). Fungal succession and lignin decomposition on Shorea obtusa leaves in a tropical seasonal forest in northern Thailand. Fungal Diversity,36, 101-119.
- SÁNCHEZ MÁRQUEZ, S., BILLS, G.F., ACUÑA, L.D.,ZABALGOGEAZCOA, I. (2010). Endophytic mycobiota of leaves and roots of the grass Holcus lanatus. Fungal Diversity, 41, 115-123.
- SCHULZ, B., BOYLE, C., DRAEGER, S., ROMMERT, A.,KROHN, K. (2002). Endophytic fungi: a source of novel biologically active secondary metabolites. Mycological Research, 106, 996-1004.
- SCHULZ, B.,BOYLE, C. (2005). The endophytic continuum. Mycological Research, 109, 6, 661-686. STONE, J.K., BACON, C.W.,WHITE, J.F. (2000). An overview of endophytic microbes: endophytism defined. In: Bacon, C.W., White, J.F.J. (Eds.), Microbial Endophytes. CRC Press, 3-29.
- STROBEL, G.,DAISY, B. (2003). Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiology and Molecular Biology Reviews, 67, 4, 491-502.
- WANG, Y., GAO, B.L., LI, X.X., ZHANG, Z. BIN, YAN, R.M., YANG, H.L.,ZHU, D. (2015). Phylogenetic diversity of culturable endophytic fungi in Dongxiang wild rice (Oryza rufipogon Griff), detection of polyketide synthase gene and their antagonistic activity analysis. Fungal Biology,119, 1032-1045