Random Lasing Detection of Mutant Huntingtin Expression in Cells

  1. Fumagallo-Reading, Felipe
  2. González-Hernández, Tomás
  3. Lahoz, Fernando
  4. de Armas-Rillo, Sergio
  5. Luis-Ravelo, Diego
  6. Abdul-Jalbar, Beatriz
  1. 1 Universidad de La Laguna
    info

    Universidad de La Laguna

    San Cristobal de La Laguna, España

    ROR https://ror.org/01r9z8p25

Journal:
Sensors

ISSN: 1424-8220

Year of publication: 2021

Volume: 21

Issue: 11

Pages: 3825

Type: Article

DOI: 10.3390/S21113825 GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Sensors

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Abstract

Huntington’s disease (HD) is an autosomal dominant, incurable neurodegenerative disease caused by mutation in the huntingtin gene (HTT). HTT mutation leads to protein misfolding and aggregation, which affect cells’ functions and structural features. Because these changes might modify the scattering strength of affected cells, we propose that random lasing (RL) is an appropriate technique for detecting cells that express mutated HTT. To explore this hypothesis, we used a cell model of HD based on the expression of two different forms—pathogenic and non-pathogenic—of HTT. The RL signals from both cell profiles were compared. A multivariate statistical analysis of the RL signals based on the principal component analysis (PCA) and linear discriminant analysis (LDA) techniques revealed substantial differences between cells that expressed the pathogenic and the non-pathogenic forms of HTT.

Bibliographic References

  • 10.1109/JQE.1966.1074123
  • Letokhov, (1968), Sov. J. Exp. Theor. Phys., 26, pp. 835
  • 10.1063/1.122853
  • 10.1103/PhysRevA.64.063808
  • 10.1038/nphoton.2012.90
  • 10.1103/PhysRevE.54.4256
  • 10.1038/nphys971
  • Cao, (2007), Opt. Prop. Nanostruct. Random Media, 330, pp. 303
  • 10.1016/j.orgel.2012.06.029
  • 10.1063/1.4823783
  • 10.1364/BOE.10.000807
  • 10.1088/1612-2011/12/4/045805
  • 10.1364/BOE.1.001401
  • 10.1364/OL.35.001425
  • 10.1016/j.orgel.2019.105389
  • 10.1063/1.1782259
  • 10.1038/s41598-017-08625-3
  • 10.1021/acssensors.8b01188
  • 10.1016/S1474-4422(13)70088-7
  • 10.1007/s12035-014-8932-1
  • 10.1038/nature02998
  • 10.1016/j.neuron.2019.01.039
  • 10.1007/s12035-015-9662-8
  • 10.1038/nrdp.2015.5
  • 10.3233/JHD-170270
  • 10.1136/jnnp.2007.128728
  • 10.3389/fnagi.2014.00078
  • 10.1056/NEJMoa1900907
  • 10.3233/JHD-170273
  • 10.1016/j.mcn.2019.02.004
  • 10.1016/S1474-4422(20)30143-5
  • 10.1177/001316446002000116
  • 10.1088/1612-2011/11/4/046001
  • 10.1039/C9SC03711J
  • Matthes, (2013), Health Phys., 105, pp. 271, 10.1097/HP.0b013e3182983fd4
  • 10.1021/nl400448n