Supercritical CO2 technology for one-pot foaming and sterilization of polymeric scaffolds for bone regeneration

  1. García-González, Carlos A.
  2. Santos-Rosales, Víctor
  3. Magariños, Beatriz
  4. Suárez-González, Javier 1
  5. Fariña, José B. 1
  6. Starbird, Ricardo
  7. Alvarez-Lorenzo, Carmen
  1. 1 Universidad de La Laguna
    info

    Universidad de La Laguna

    San Cristobal de La Laguna, España

    GRID grid.10041.34

Journal:
International Journal of Pharmaceutics

ISSN: 0378-5173

Year of publication: 2021

Volume: 605

Pages: 120801

Type: Article

Export: RIS
DOI: 10.1016/j.ijpharm.2021.120801 GOOGLE SCHOLAR

Metrics

Cited by

  • Scopus Cited by: 0 (22-11-2021)

JCR (Journal Impact Factor)

(Indicator corresponding to the last year available on this portal, year 2020)
  • Year 2020
  • Journal Impact Factor: 5.875
  • Best Quartile: Q1
  • Area: PHARMACOLOGY & PHARMACY Quartile: Q1 Rank in area: 37/276 (Ranking edition: SCIE)

SCImago Journal Rank

(Indicator corresponding to the last year available on this portal, year 2020)
  • Year 2020
  • SJR Journal Impact: 1.153
  • Best Quartile: Q1
  • Area: Pharmaceutical Science Quartile: Q1 Rank in area: 13/182

CiteScore

(Indicator corresponding to the last year available on this portal, year 2020)
  • Year 2020
  • CiteScore of the Journal : 8.2
  • Area: Pharmaceutical Science Percentile: 92

Abstract

Sterilization is a quite challenging step in the development of novel polymeric scaffolds for regenerative medicine since conventional sterilization techniques may significantly alter their morphological and physicochemical properties. Supercritical (sc) sterilization, i.e. the use of scCO2 as a sterilizing agent, emerges as a promising sterilization method due to the mild operational conditions and excellent penetration capability. In this work, a scCO2 protocol was implemented for the one-pot preparation and sterilization of poly(-caprolactone) (PCL)/poly(lactic-co-glycolic acid) (PLGA) scaffolds. The sterilization conditions were established after screening against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) vegetative bacteria and spores of Bacillus stearothermophilus, Bacillus pumilus and Bacillus atrophaeus. The transition from the sterilization conditions (140 bar, 39 °C) to the compressed foaming (60 bar, 26 °C) was performed through controlled depressurization (3.2 bar/min) and CO2 liquid flow. Controlled depressurization/pressurization cycles were subsequently applied. Using this scCO2 technology toolbox, sterile scaffolds of well-controlled pore architecture were obtained. This sterilization procedure successfully achieved not only SAL-6 against well-known resistant bacteria endospores but also improved the scaffold morphologies compared to standard gamma radiation sterilization procedures.

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