Early Transcriptional Liver Signatures in Experimental Visceral Leishmaniasis
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Dorta-Guerra, Roberto
1
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Carmelo, Emma
1
- Diaz-Solano, Raquel
- Palacios, Génesis 1
- Valladares, Basilio 1
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1
Universidad de La Laguna
info
ISSN: 1422-0067
Year of publication: 2021
Volume: 22
Issue: 13
Pages: 7161
Type: Article
More publications in: International Journal of Molecular Sciences
Metrics
JCR (Journal Impact Factor)
- Year 2021
- Journal Impact Factor: 6.208
- Journal Impact Factor without self cites: 5.608
- Article influence score: 1.064
- Best Quartile: Q1
- Area: BIOCHEMISTRY & MOLECULAR BIOLOGY Quartile: Q1 Rank in area: 69/297 (Ranking edition: SCIE)
- Area: CHEMISTRY, MULTIDISCIPLINARY Quartile: Q2 Rank in area: 50/179 (Ranking edition: SCIE)
SCImago Journal Rank
- Year 2021
- SJR Journal Impact: 1.176
- Best Quartile: Q1
- Area: Inorganic Chemistry Quartile: Q1 Rank in area: 8/68
- Area: Organic Chemistry Quartile: Q1 Rank in area: 19/180
- Area: Spectroscopy Quartile: Q1 Rank in area: 6/75
- Area: Physical and Theoretical Chemistry Quartile: Q1 Rank in area: 23/174
- Area: Computer Science Applications Quartile: Q1 Rank in area: 128/729
- Area: Medicine (miscellaneous) Quartile: Q1 Rank in area: 343/2489
- Area: Catalysis Quartile: Q2 Rank in area: 17/57
- Area: Molecular Biology Quartile: Q2 Rank in area: 140/405
Scopus CiteScore
- Year 2021
- CiteScore of the Journal : 6.9
- Area: Inorganic Chemistry Percentile: 85
- Area: Spectroscopy Percentile: 85
- Area: Physical and Theoretical Chemistry Percentile: 83
- Area: Computer Science Applications Percentile: 81
- Area: Organic Chemistry Percentile: 80
- Area: Molecular Biology Percentile: 61
- Area: Catalysis Percentile: 58
Journal Citation Indicator (JCI)
- Year 2021
- Journal Citation Indicator (JCI): 0.7
- Best Quartile: Q2
- Area: CHEMISTRY, MULTIDISCIPLINARY Quartile: Q2 Rank in area: 77/224
- Area: BIOCHEMISTRY & MOLECULAR BIOLOGY Quartile: Q2 Rank in area: 154/322
Abstract
Transcriptional analysis of complex biological scenarios has been used extensively, even though sometimes the results of such analysis may prove imprecise or difficult to interpret due to an overwhelming amount of information. In this study, a large-scale real-time qPCR experiment was coupled to multivariate statistical analysis in order to describe the main immunological events underlying the early L. infantum infection in livers of BALB/c mice. High-throughput qPCR was used to evaluate the expression of 223 genes related to immunological response and metabolism 1, 3, 5, and 10 days post infection. This integrative analysis showed strikingly different gene signatures at 1 and 10 days post infection, revealing the progression of infection in the experimental model based on the upregulation of particular immunological response patterns and mediators. The gene signature 1 day post infection was not only characterized by the upregulation of mediators involved in interferon signaling and cell chemotaxis, but also the upregulation of some inhibitory markers. In contrast, at 10 days post infection, the upregulation of many inflammatory and Th1 markers characterized a more defined gene signature with the upregulation of mediators in the IL-12 signaling pathway. Our results reveal a significant connection between the expression of innate immune response and metabolic and inhibitory markers in early L. infantum infection of the liver.
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