Circuitos cerebrales implicados en la fisiopatologia de la enfermedad de parkinsonvia talamoestriada y expresion de trasportadores vesiculares de glutamato

Resumen

#TITULO: CIRCUITOS CEREBRALES IMPLICADOS EN LA FISIOPATOLOGIA DE LA ENFERMEDAD DE PARKINSON: VIA TALAMOESTRIADA Y EXPRESIÓN DE TRANSPORTADORES VESICULARES DE GLUTAMATO #RESUMEN: Cerebral circuits engaged in the pathophysiology of Parkinson's disease: Thalamostriatal pathway and expression of vesicular glutamate transporters. This work is focused on analyzing the potential impact of the unilateral dopaminergic depletion on the thalamostriatal pathway of rats treated with 6-OHDA, as well as to address the distribution of VGLUTl and VGLUT2 mRNAs in throdent thalamus. The role of the centromedian-parafascicular complex (CM-Pf) on the pathophysiology of Parkinson's disease (PD) has gained increased interest over the past few years. on one hand, the presence of neurodeqenerative phenomena within CM-Pf has been made available recently. On the other hand, a number of studies have demonstrated increased metabolic activity of CM-Pf in animal models of PD and therefore this point can lead to the consideration of CM-Pf as a target for functional neurosurgery of movement disorders of basal ganglia on'gin. At present, deep brain stimulation (DBS) of the subthalamic nucleus (STN) is widely accepted as the gold standard for long-term suffering PD patients. The increased activity of CM-Pf has paved the way for considering this thalamic nucleus as a potential surgical target, although the ongoing degenerative phenomena within CM-Pf could challenge this way of thinking. Data gathered from our studies demonstrated marked neurodegenerative changes within the CM-Pf neurons giving rise to thalamostriatal proiections. By contrast, surviving neurons became clearly hyperactive, probably as a consequence of self-compensating phenomena. Secondarily, our studies addressed the distribution of two isoforms of the vesicular glutamate transporter (VGLUTl and VGLUT2) within the rodent thalamus. Our results established a new map of VGLUTs distribution, with overlap of both isoforms in all the nuclei except for the intralaminar and midline nuclei, which are selective for VGLUT2 mRNA expression.