Micropropagation of Helianthemum inaguae, a rare and endangered species from the Canary Islands.

  1. Santana López, Isabel
  2. Valdés González, Francisco
  3. Luis Jorge, Juan Cristo
Journal:
Botánica Macaronésica

ISSN: 0211-7150

Year of publication: 2006

Issue: 26

Pages: 55-64

Type: Article

More publications in: Botánica Macaronésica

Abstract

A micropropagation protocol has been developed for Helianthemum inaguae Marrero Rodr., González Martín & González Artiles, an endangered species from Gran Canaria (Canary Islands, Spain). Shoot tips and nodal segments isolated from in vitro germinated seedlings, aged two months, were used as primary explants. Multiple shoot production was obtained using MS medium supplemented with different concentrations of BA, Kin and NAA, showing BA and Kin the best results. Finally, rooting was achieved also in MS medium by adding IBA or without any plant growth regulator. A 72% survival rate was obtained during the acclimatisation process.