Injectable Scaffold for Bone Marrow Stem Cells and Bone Morphogenetic Protein-2 to Repair Cartilage
- Arnau, María Rosa 1
- Vayas, Raquel 23
- Delgado, Araceli 24
- Reyes, Ricardo 45
- Évora, Carmen 24
- 1 Servicio de Estabulario y Animalario del Servicio General de Apoyo a la Investigación, Universidad de La Laguna, La Laguna, Spain
- 2 Department of Chemical Engineering and Pharmaceutical Technology, Universidad de La Laguna, La Laguna, Spain
- 3 Servicio de Cirugía Ortopédica y Traumatología, Complejo Hospitalario Universitario Ntra, Sra. de Candelaria, Santa Cruz de Tenerife, Spain
- 4 Institute of Biomedical Technologies, Center for Biomedical Research of the Canary Islands, Universidad de La Laguna, La Laguna, Spain
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5
Universidad de La Laguna
info
ISSN: 1947-6035, 1947-6043
Year of publication: 2019
Pages: 293-306
Type: Article
More publications in: Cartilage
Sustainable development goals
Abstract
Objective: The limits of the microfracture (MFX) treatment in terms of lesion size and long-term tissue functionality makes it necessary to investigate different alternatives to repair focal cartilage lesions. The present study aims at evaluating the efficacy of a minimally invasive approach against the conventional MFX to repair a chondral defect in rabbits. An injectable scaffold of BMP-2 pre-encapsulated in PLGA microspheres dispersed in a Pluronic F-127 solution is proposed as support of cells and controlled delivery system for the growth factor.Design: MFX was compared versus the injectable system seeded with mesenchymal stem cells (MSCs), both without BMP-2 and under controlled release of BMP-2 at 2 different doses (3 and 12 µg/scaffold). The different treatments were evaluated on a 4-mm diameter chondral defect model using 9 experimental groups of 4 rabbits (8 knees) each, throughout 24 weeks.Results: Histologically, all the treated groups, except MFX treated, responded significantly better than the control group (nontreated defect). Although no significant differences were found between the treated groups, only BMP(12), MSC-BMP(12), and MFX-BMP(3) groups showed nonsignificant differences when compared with the normal cartilage.Conclusions: The hydrogel system proposed to control the release rate of the BMP-2 was safe, easily injectable, and also provided good support for cells. Treatments with MSCs or BMP-2 repaired efficiently the chondral lesion created in rabbits, being less invasive than MFX treatment.
Bibliographic References
- 10.1053/j.otsm.2013.03.001
- 10.1249/MSS.0b013e3181d9eea0
- 10.1634/stemcells.2006-0311
- 10.1016/j.arthro.2014.11.031
- 10.5435/JAAOS-22-07-467
- 10.5435/00124635-200311000-00006
- 10.1007/s00167-012-2256-3
- 10.1016/j.joca.2013.08.024
- 10.1089/ten.tea.2012.0233
- 10.1016/j.knee.2013.04.003
- 10.1016/j.knee.2014.10.003
- 10.1016/j.joca.2006.05.003
- 10.2106/00004623-200311000-00008
- 10.2106/00004623-200300003-00017
- 10.1002/1097-4644(20010501)81:2<368::AID-JCB1051>3.0.CO;2-J
- 10.1007/s11999-011-1857-3
- 10.1016/j.joca.2008.03.003
- 10.5435/JAAOS-21-05-303
- 10.1016/j.actbio.2011.10.008
- 10.1088/1748-6041/10/4/045008
- 10.1016/j.jconrel.2006.05.026
- 10.22203/eCM.v025a25
- 10.1007/s00264-009-0818-x
- 10.1016/j.ejps.2013.06.008
- 10.1177/0363546510363433
- 10.1007/s00167-011-1596-8
- 10.1186/s13075-015-0537-1
- 10.1016/j.biomaterials.2009.09.009
- 10.3390/ijms161125937
- 10.1002/stem.1890
- Reyes R, (2014), J Tissue Eng Regen Med, 8, pp. 521
- 10.1002/jbm.a.34769
- 10.1088/1748-605X/aa6f1c
- 10.5966/sctm.2016-0020
- 10.1039/C4BM00431K
- 10.1371/journal.pone.0144252
- 10.1089/ten.tec.2013.0724
- 10.2106/00004623-199605000-00012
- 10.1016/j.injury.2010.09.027
- 10.1089/ten.tea.2014.0384
- 10.1007/s00132-017-3491-6
- 10.1007/s00167-008-0522-1
- 10.2106/JBJS.I.01284
- 10.3928/01477447-20120426-20
- 10.1302/0301-620X.92B8.24341
- 10.1007/s11999-011-2107-4
- 10.3727/096368909X12483162197169
- 10.1007/s11033-011-0853-8