Injectable Scaffold for Bone Marrow Stem Cells and Bone Morphogenetic Protein-2 to Repair Cartilage

  1. Arnau, María Rosa 1
  2. Vayas, Raquel 23
  3. Delgado, Araceli 24
  4. Reyes, Ricardo 45
  5. Évora, Carmen 24
  1. 1 Servicio de Estabulario y Animalario del Servicio General de Apoyo a la Investigación, Universidad de La Laguna, La Laguna, Spain
  2. 2 Department of Chemical Engineering and Pharmaceutical Technology, Universidad de La Laguna, La Laguna, Spain
  3. 3 Servicio de Cirugía Ortopédica y Traumatología, Complejo Hospitalario Universitario Ntra, Sra. de Candelaria, Santa Cruz de Tenerife, Spain
  4. 4 Institute of Biomedical Technologies, Center for Biomedical Research of the Canary Islands, Universidad de La Laguna, La Laguna, Spain
  5. 5 Universidad de La Laguna
    info

    Universidad de La Laguna

    San Cristobal de La Laguna, España

    GRID grid.10041.34

Journal:
Cartilage

ISSN: 1947-6035

Year of publication: 2019

Pages: 194760351984168

Type: Article

Export: RIS
DOI: 10.1177/1947603519841682 GOOGLE SCHOLAR

Metrics

Cited by

  • Scopus Cited by: 3 (18-11-2021)

JCR (Journal Impact Factor)

  • Year 2019
  • Journal Impact Factor: 3.857
  • Best Quartile: Q1
  • Area: ORTHOPEDICS Quartile: Q1 Rank in area: 9/82 (Ranking edition: SCIE)

SCImago Journal Rank

  • Year 2019
  • SJR Journal Impact: 0.922
  • Best Quartile: Q1
  • Area: Physical Therapy, Sports Therapy and Rehabilitation Quartile: Q1 Rank in area: 36/207
  • Area: Biomedical Engineering Quartile: Q2 Rank in area: 65/324
  • Area: Immunology and Allergy Quartile: Q2 Rank in area: 96/202

CiteScore

  • Year 2019
  • CiteScore of the Journal : 4.8
  • Area: Physical Therapy, Sports Therapy and Rehabilitation Percentile: 91
  • Area: Biomedical Engineering Percentile: 70
  • Area: Immunology and Allergy Percentile: 54

Journal Citation Indicator (JCI)

  • Year 2019
  • Journal Citation Indicator (JCI): 1.22
  • Best Quartile: Q1
  • Area: ORTHOPEDICS Quartile: Q1 Rank in area: 15/81

Abstract

Objective: The limits of the microfracture (MFX) treatment in terms of lesion size and long-term tissue functionality makes it necessary to investigate different alternatives to repair focal cartilage lesions. The present study aims at evaluating the efficacy of a minimally invasive approach against the conventional MFX to repair a chondral defect in rabbits. An injectable scaffold of BMP-2 pre-encapsulated in PLGA microspheres dispersed in a Pluronic F-127 solution is proposed as support of cells and controlled delivery system for the growth factor.Design: MFX was compared versus the injectable system seeded with mesenchymal stem cells (MSCs), both without BMP-2 and under controlled release of BMP-2 at 2 different doses (3 and 12 µg/scaffold). The different treatments were evaluated on a 4-mm diameter chondral defect model using 9 experimental groups of 4 rabbits (8 knees) each, throughout 24 weeks.Results: Histologically, all the treated groups, except MFX treated, responded significantly better than the control group (nontreated defect). Although no significant differences were found between the treated groups, only BMP(12), MSC-BMP(12), and MFX-BMP(3) groups showed nonsignificant differences when compared with the normal cartilage.Conclusions: The hydrogel system proposed to control the release rate of the BMP-2 was safe, easily injectable, and also provided good support for cells. Treatments with MSCs or BMP-2 repaired efficiently the chondral lesion created in rabbits, being less invasive than MFX treatment.

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