Injectable Scaffold for Bone Marrow Stem Cells and Bone Morphogenetic Protein-2 to Repair Cartilage
- Arnau, María Rosa 1
- Vayas, Raquel 23
- Delgado, Araceli 24
-
Reyes, Ricardo
45
- Évora, Carmen 24
- 1 Servicio de Estabulario y Animalario del Servicio General de Apoyo a la Investigación, Universidad de La Laguna, La Laguna, Spain
- 2 Department of Chemical Engineering and Pharmaceutical Technology, Universidad de La Laguna, La Laguna, Spain
- 3 Servicio de Cirugía Ortopédica y Traumatología, Complejo Hospitalario Universitario Ntra, Sra. de Candelaria, Santa Cruz de Tenerife, Spain
- 4 Institute of Biomedical Technologies, Center for Biomedical Research of the Canary Islands, Universidad de La Laguna, La Laguna, Spain
-
5
Universidad de La Laguna
info
ISSN: 1947-6035, 1947-6043
Año de publicación: 2019
Páginas: 293-306
Tipo: Artículo
Otras publicaciones en: Cartilage
Resumen
Objective: The limits of the microfracture (MFX) treatment in terms of lesion size and long-term tissue functionality makes it necessary to investigate different alternatives to repair focal cartilage lesions. The present study aims at evaluating the efficacy of a minimally invasive approach against the conventional MFX to repair a chondral defect in rabbits. An injectable scaffold of BMP-2 pre-encapsulated in PLGA microspheres dispersed in a Pluronic F-127 solution is proposed as support of cells and controlled delivery system for the growth factor.Design: MFX was compared versus the injectable system seeded with mesenchymal stem cells (MSCs), both without BMP-2 and under controlled release of BMP-2 at 2 different doses (3 and 12 µg/scaffold). The different treatments were evaluated on a 4-mm diameter chondral defect model using 9 experimental groups of 4 rabbits (8 knees) each, throughout 24 weeks.Results: Histologically, all the treated groups, except MFX treated, responded significantly better than the control group (nontreated defect). Although no significant differences were found between the treated groups, only BMP(12), MSC-BMP(12), and MFX-BMP(3) groups showed nonsignificant differences when compared with the normal cartilage.Conclusions: The hydrogel system proposed to control the release rate of the BMP-2 was safe, easily injectable, and also provided good support for cells. Treatments with MSCs or BMP-2 repaired efficiently the chondral lesion created in rabbits, being less invasive than MFX treatment.
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